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Navigate SMGW and perform the steps as follow:
Click on menu tab Goto->Expert Functions->External Security->Maintenance of ACL Files

 

 

Steps to reread RFC Gateway ACL files:

SMGW->goto->Expert Functions->External Security->Maintenance of ACL files. (here you will be able to see RFC Gateway ACL files) ->goto ->reread/reread(global)

If you have done the changes in ACL files you will see the following prompt

You can click ok/cancel to close the popup. Perform the above mentioned steps in popup  to reread the ACL files.

 

You can also check the following help link:

https://help.sap.com/doc/62b4de4187cb43668d15dac48fc00732/7.5.8/en-US/5872aed2b8cc4b0387374cbf5f4adcf6.html